2014 pcmv mcherry cry2clust addgene (Addgene inc)
93
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Addgene inc
2014 pcmv mcherry cry2clust addgene
2014 Pcmv Mcherry Cry2clust Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2014+addgene+plasmid/mCherry-CRY2clust+(Plasmid+%23105624)/pm41549960-464-9-11
Average 93 stars, based on 13 article reviews
2014 Pcmv Mcherry Cry2clust Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2014+addgene+plasmid/mCherry-CRY2clust+(Plasmid+%23105624)/pm41549960-464-9-11
Average 93 stars, based on 13 article reviews
2014 pcmv mcherry cry2clust addgene - by Bioz Stars,
2026-10
93/100 stars
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Recombinant:Article Title: Kinetochore-fiber lengths are maintained locally but coordinated globally by poles in the mammalian spindle Article Snippet: .. Progenitors: Addgene #114021 (Geert Kops) and mCherry- p50 (PMID:19196984) Recombinant DNA reagent eGFP-α-tubulin (plasmid) Michael Davidson collection given to UCSF; Rizzo et al., 2009 Addgene Plasmid #56450 Recombinant DNA reagent mCherry- p50 (plasmid) Gift from M. Meffert, Johns Hopkins University; PMID:19196984 Recombinant DNA reagent β-Tubulin HaloTag (plasmid) Addgene; Uno et al., Article Title: Immunity of Heterologously and Homologously Boosted or Convalescent Individuals Against Omicron BA.1, BA.2, and BA.4/5 Variants. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SARS-CoV-2 RBD neutralizing antibody rabbit mAb Sino Biological Cat # 40592-R001; RRID: AB_2857936 ACE2 Monoclonal antibody Mouse Proteintech Cat # 66699-1-Ig; RRID: AB_2882052 SARS-CoV-2 Spike RBD Mouse mAb ABclonal Cat # A20141 HRP-conjugated Goat AntiMouse IgG(H+L) Proteintech Cat # SA00001-1; RRID: AB_2722565 Mouse anti-b-actin mAb Proteintech Cat # 66009-1-Ig; RRID: AB_2687938 Rhodamine (TRITC)–conjugated Goat AntiMouse IgG(H+L) Proteintech Cat # SA00007-1; RRID: AB_2889940 Chemicals, peptides, and recombinant proteins Hoechst 33342 ThermoFisher Cat # 62249 8-well Nunc Lab-Tek ThermoFisher Cat # 155411 Lipofectamine 3000 ThermoFisher Cat # L3000008 Polyethylenimine MAX 40K Polysciences Cat # 24765 Expression medium Sino Biological Cat # SMM 293-TI cell culture supplement Sino Biological Cat # M293-SUPI SNAP-Cy3 New England BioLabs Cat # S9112S SNAP-Alexa 488 New England BioLabs Cat # S9129S Lyso561 KeyGEN BioTECH Cat # KGMP006 Bafilomycin A1 MedChemExpress Cat # HY-100558 MCA-APK(Dnp)-OH Beyotime Cat # P9737 Dynasore TargetMOI Cat # T1848 HCQ Sigma-Aldrich Cat # H0915 CQ Innochem Cat # A88133 Experimental models: Cell lines Hela cells ATCC Cat #CCL-2 HEK293T ATCC Cat #CRL3216 NCl-H1299 ATCC Cat # CRL-5803 Vero ATCC Cat #CCL-81 Caco-2 ATCC Cat #HTB-37 Expi293F Cells ThermoFisher Cat #A14527 Recombinant DNA mCherry-Rab7A Rowland et al. Article Title: TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages. Article Snippet: .. GCACATGTCG This manuscript PB-REV Recombinant DNA p5E-ubi Mosimann et al., 2011 Addgene Plasmid #27320; RRID: Addgene_27320 pDestTol2pA2 Kwan et al., 2007 #394 pME-MCS Kwan et al., 2007 #237 p3E-MCS Don et al., 2017 Addgene Plasmid #75174; RRID: Addgene_75174 ZebraF-Cassa-#3 Garcia-Marques et al., 2019 ZebraF-Cassa-#3 pME-H2B-mCerulean3 Don et al., 2017 Addgene plasmid #75156; RRID: Addgene_75156 pToneDest (pDesTol1) Shin et al., 2016 Addgene plasmid #67691; RRID: Addgene_67691 p5E-5xUAS vector Asakawa et al., 2008 p5E-5xUAS pME-zCas9 Ablain et al., 2015 Addgene plasmid # 63154; RRID: Addgene_63154 p5E-her4.1 Yeo et al., 2007 p5E-her4.1 pXL-CMV-piggyBac Transposagen Biopharmaceuticals Inc N/A pcDNA3.1/Puro-CAG-ASAP1 St-Pierre et al., Article Title: Reciprocal inhibition of NOTCH and SOX2 shapes tumor cell plasticity and therapeutic escape in triple-negative breast cancer. Article Snippet: Cancer cell plasticity contributes significantly to the failure of chemoand targeted therapies in triple-negative breast cancer (TNBC).. Molecular mechanisms of therapy-induced tumor cell plasticity and associated resistance are largely unknown.. Using a genome-wide CRISPR-Cas9 screen, we investigated escape mechanisms of NOTCH-driven TNBC treated with a gammasecretase inhibitor (GSI) and identified SOX2 as a target of resistance to Notch inhibition. Article Title: Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model. Article Snippet: Article Plasmid Preparation:Article Title: Kinetochore-fiber lengths are maintained locally but coordinated globally by poles in the mammalian spindle Article Snippet: .. Progenitors: Addgene #114021 (Geert Kops) and mCherry- p50 (PMID:19196984) Recombinant DNA reagent eGFP-α-tubulin (plasmid) Michael Davidson collection given to UCSF; Rizzo et al., 2009 Addgene Plasmid #56450 Recombinant DNA reagent mCherry- p50 (plasmid) Gift from M. Meffert, Johns Hopkins University; PMID:19196984 Recombinant DNA reagent β-Tubulin HaloTag (plasmid) Addgene; Uno et al., Article Title: Immunity of Heterologously and Homologously Boosted or Convalescent Individuals Against Omicron BA.1, BA.2, and BA.4/5 Variants. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SARS-CoV-2 RBD neutralizing antibody rabbit mAb Sino Biological Cat # 40592-R001; RRID: AB_2857936 ACE2 Monoclonal antibody Mouse Proteintech Cat # 66699-1-Ig; RRID: AB_2882052 SARS-CoV-2 Spike RBD Mouse mAb ABclonal Cat # A20141 HRP-conjugated Goat AntiMouse IgG(H+L) Proteintech Cat # SA00001-1; RRID: AB_2722565 Mouse anti-b-actin mAb Proteintech Cat # 66009-1-Ig; RRID: AB_2687938 Rhodamine (TRITC)–conjugated Goat AntiMouse IgG(H+L) Proteintech Cat # SA00007-1; RRID: AB_2889940 Chemicals, peptides, and recombinant proteins Hoechst 33342 ThermoFisher Cat # 62249 8-well Nunc Lab-Tek ThermoFisher Cat # 155411 Lipofectamine 3000 ThermoFisher Cat # L3000008 Polyethylenimine MAX 40K Polysciences Cat # 24765 Expression medium Sino Biological Cat # SMM 293-TI cell culture supplement Sino Biological Cat # M293-SUPI SNAP-Cy3 New England BioLabs Cat # S9112S SNAP-Alexa 488 New England BioLabs Cat # S9129S Lyso561 KeyGEN BioTECH Cat # KGMP006 Bafilomycin A1 MedChemExpress Cat # HY-100558 MCA-APK(Dnp)-OH Beyotime Cat # P9737 Dynasore TargetMOI Cat # T1848 HCQ Sigma-Aldrich Cat # H0915 CQ Innochem Cat # A88133 Experimental models: Cell lines Hela cells ATCC Cat #CCL-2 HEK293T ATCC Cat #CRL3216 NCl-H1299 ATCC Cat # CRL-5803 Vero ATCC Cat #CCL-81 Caco-2 ATCC Cat #HTB-37 Expi293F Cells ThermoFisher Cat #A14527 Recombinant DNA mCherry-Rab7A Rowland et al. Article Title: TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages. Article Snippet: .. GCACATGTCG This manuscript PB-REV Recombinant DNA p5E-ubi Mosimann et al., 2011 Addgene Plasmid #27320; RRID: Addgene_27320 pDestTol2pA2 Kwan et al., 2007 #394 pME-MCS Kwan et al., 2007 #237 p3E-MCS Don et al., 2017 Addgene Plasmid #75174; RRID: Addgene_75174 ZebraF-Cassa-#3 Garcia-Marques et al., 2019 ZebraF-Cassa-#3 pME-H2B-mCerulean3 Don et al., 2017 Addgene plasmid #75156; RRID: Addgene_75156 pToneDest (pDesTol1) Shin et al., 2016 Addgene plasmid #67691; RRID: Addgene_67691 p5E-5xUAS vector Asakawa et al., 2008 p5E-5xUAS pME-zCas9 Ablain et al., 2015 Addgene plasmid # 63154; RRID: Addgene_63154 p5E-her4.1 Yeo et al., 2007 p5E-her4.1 pXL-CMV-piggyBac Transposagen Biopharmaceuticals Inc N/A pcDNA3.1/Puro-CAG-ASAP1 St-Pierre et al., Article Title: Reciprocal inhibition of NOTCH and SOX2 shapes tumor cell plasticity and therapeutic escape in triple-negative breast cancer. Article Snippet: Cancer cell plasticity contributes significantly to the failure of chemoand targeted therapies in triple-negative breast cancer (TNBC).. Molecular mechanisms of therapy-induced tumor cell plasticity and associated resistance are largely unknown.. Using a genome-wide CRISPR-Cas9 screen, we investigated escape mechanisms of NOTCH-driven TNBC treated with a gammasecretase inhibitor (GSI) and identified SOX2 as a target of resistance to Notch inhibition. Article Title: Circadian regulation of endoplasmic reticulum calcium response in cultured mouse astrocytes Article Snippet: .. resource Designation Source or reference Identifiers Additional information Gene (Mus musculus) Herpud1 (Herp) Mouse Genome Informatics MGI:1927406 Gene (M. musculus) Bmal1 Mouse Genome Informatics MGI:1096381 Gene (M. musculus) Rev- Erbα (Nr1d1) Mouse Genome Informatics MGI:2444210 Gene (M. musculus) Per2 Mouse Genome Informatics MGI:1195265 Gene (M. musculus) Itpr1 Mouse Genome Informatics MGI:96623 Gene (M. musculus) Itpr2 Mouse Genome Informatics MGI:99418 Gene (M. musculus) Cx43 (Gja1) Mouse Genome Informatics MGI:95713 Strain, strain background (M. musculus, male) C57BL/6 J Charles River Japan N/A Adult PFC experiment Strain, strain background (M. musculus, male and female) B6.129- Bmal1tm1Bra/J Jackson Laboratory Cat# 009100; RRID:IMSR_JAX:009100 Primary Bmal1 KO astrocyte culture Strain, strain background (M. musculus, male and female) 1 day old C75BL/6 N Koatch N/A Primary WT astrocyte culture Transfected construct (M. musculus) CMV- G- CEPIA1er Suzuki et al., Article Title: Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model. Article Snippet: Article Software:Article Title: Kinetochore-fiber lengths are maintained locally but coordinated globally by poles in the mammalian spindle Article Snippet: .. Progenitors: Addgene #114021 (Geert Kops) and mCherry- p50 (PMID:19196984) Recombinant DNA reagent eGFP-α-tubulin (plasmid) Michael Davidson collection given to UCSF; Rizzo et al., 2009 Addgene Plasmid #56450 Recombinant DNA reagent mCherry- p50 (plasmid) Gift from M. Meffert, Johns Hopkins University; PMID:19196984 Recombinant DNA reagent β-Tubulin HaloTag (plasmid) Addgene; Uno et al., Article Title: Immunity of Heterologously and Homologously Boosted or Convalescent Individuals Against Omicron BA.1, BA.2, and BA.4/5 Variants. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SARS-CoV-2 RBD neutralizing antibody rabbit mAb Sino Biological Cat # 40592-R001; RRID: AB_2857936 ACE2 Monoclonal antibody Mouse Proteintech Cat # 66699-1-Ig; RRID: AB_2882052 SARS-CoV-2 Spike RBD Mouse mAb ABclonal Cat # A20141 HRP-conjugated Goat AntiMouse IgG(H+L) Proteintech Cat # SA00001-1; RRID: AB_2722565 Mouse anti-b-actin mAb Proteintech Cat # 66009-1-Ig; RRID: AB_2687938 Rhodamine (TRITC)–conjugated Goat AntiMouse IgG(H+L) Proteintech Cat # SA00007-1; RRID: AB_2889940 Chemicals, peptides, and recombinant proteins Hoechst 33342 ThermoFisher Cat # 62249 8-well Nunc Lab-Tek ThermoFisher Cat # 155411 Lipofectamine 3000 ThermoFisher Cat # L3000008 Polyethylenimine MAX 40K Polysciences Cat # 24765 Expression medium Sino Biological Cat # SMM 293-TI cell culture supplement Sino Biological Cat # M293-SUPI SNAP-Cy3 New England BioLabs Cat # S9112S SNAP-Alexa 488 New England BioLabs Cat # S9129S Lyso561 KeyGEN BioTECH Cat # KGMP006 Bafilomycin A1 MedChemExpress Cat # HY-100558 MCA-APK(Dnp)-OH Beyotime Cat # P9737 Dynasore TargetMOI Cat # T1848 HCQ Sigma-Aldrich Cat # H0915 CQ Innochem Cat # A88133 Experimental models: Cell lines Hela cells ATCC Cat #CCL-2 HEK293T ATCC Cat #CRL3216 NCl-H1299 ATCC Cat # CRL-5803 Vero ATCC Cat #CCL-81 Caco-2 ATCC Cat #HTB-37 Expi293F Cells ThermoFisher Cat #A14527 Recombinant DNA mCherry-Rab7A Rowland et al. Article Title: Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model. Article Snippet: Article Expressing:Article Title: Immunity of Heterologously and Homologously Boosted or Convalescent Individuals Against Omicron BA.1, BA.2, and BA.4/5 Variants. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SARS-CoV-2 RBD neutralizing antibody rabbit mAb Sino Biological Cat # 40592-R001; RRID: AB_2857936 ACE2 Monoclonal antibody Mouse Proteintech Cat # 66699-1-Ig; RRID: AB_2882052 SARS-CoV-2 Spike RBD Mouse mAb ABclonal Cat # A20141 HRP-conjugated Goat AntiMouse IgG(H+L) Proteintech Cat # SA00001-1; RRID: AB_2722565 Mouse anti-b-actin mAb Proteintech Cat # 66009-1-Ig; RRID: AB_2687938 Rhodamine (TRITC)–conjugated Goat AntiMouse IgG(H+L) Proteintech Cat # SA00007-1; RRID: AB_2889940 Chemicals, peptides, and recombinant proteins Hoechst 33342 ThermoFisher Cat # 62249 8-well Nunc Lab-Tek ThermoFisher Cat # 155411 Lipofectamine 3000 ThermoFisher Cat # L3000008 Polyethylenimine MAX 40K Polysciences Cat # 24765 Expression medium Sino Biological Cat # SMM 293-TI cell culture supplement Sino Biological Cat # M293-SUPI SNAP-Cy3 New England BioLabs Cat # S9112S SNAP-Alexa 488 New England BioLabs Cat # S9129S Lyso561 KeyGEN BioTECH Cat # KGMP006 Bafilomycin A1 MedChemExpress Cat # HY-100558 MCA-APK(Dnp)-OH Beyotime Cat # P9737 Dynasore TargetMOI Cat # T1848 HCQ Sigma-Aldrich Cat # H0915 CQ Innochem Cat # A88133 Experimental models: Cell lines Hela cells ATCC Cat #CCL-2 HEK293T ATCC Cat #CRL3216 NCl-H1299 ATCC Cat # CRL-5803 Vero ATCC Cat #CCL-81 Caco-2 ATCC Cat #HTB-37 Expi293F Cells ThermoFisher Cat #A14527 Recombinant DNA mCherry-Rab7A Rowland et al. Cell Culture:Article Title: Immunity of Heterologously and Homologously Boosted or Convalescent Individuals Against Omicron BA.1, BA.2, and BA.4/5 Variants. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SARS-CoV-2 RBD neutralizing antibody rabbit mAb Sino Biological Cat # 40592-R001; RRID: AB_2857936 ACE2 Monoclonal antibody Mouse Proteintech Cat # 66699-1-Ig; RRID: AB_2882052 SARS-CoV-2 Spike RBD Mouse mAb ABclonal Cat # A20141 HRP-conjugated Goat AntiMouse IgG(H+L) Proteintech Cat # SA00001-1; RRID: AB_2722565 Mouse anti-b-actin mAb Proteintech Cat # 66009-1-Ig; RRID: AB_2687938 Rhodamine (TRITC)–conjugated Goat AntiMouse IgG(H+L) Proteintech Cat # SA00007-1; RRID: AB_2889940 Chemicals, peptides, and recombinant proteins Hoechst 33342 ThermoFisher Cat # 62249 8-well Nunc Lab-Tek ThermoFisher Cat # 155411 Lipofectamine 3000 ThermoFisher Cat # L3000008 Polyethylenimine MAX 40K Polysciences Cat # 24765 Expression medium Sino Biological Cat # SMM 293-TI cell culture supplement Sino Biological Cat # M293-SUPI SNAP-Cy3 New England BioLabs Cat # S9112S SNAP-Alexa 488 New England BioLabs Cat # S9129S Lyso561 KeyGEN BioTECH Cat # KGMP006 Bafilomycin A1 MedChemExpress Cat # HY-100558 MCA-APK(Dnp)-OH Beyotime Cat # P9737 Dynasore TargetMOI Cat # T1848 HCQ Sigma-Aldrich Cat # H0915 CQ Innochem Cat # A88133 Experimental models: Cell lines Hela cells ATCC Cat #CCL-2 HEK293T ATCC Cat #CRL3216 NCl-H1299 ATCC Cat # CRL-5803 Vero ATCC Cat #CCL-81 Caco-2 ATCC Cat #HTB-37 Expi293F Cells ThermoFisher Cat #A14527 Recombinant DNA mCherry-Rab7A Rowland et al. Article Title: Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model. Article Snippet: Article Western Blot:Article Title: Reciprocal inhibition of NOTCH and SOX2 shapes tumor cell plasticity and therapeutic escape in triple-negative breast cancer. Article Snippet: Cancer cell plasticity contributes significantly to the failure of chemoand targeted therapies in triple-negative breast cancer (TNBC).. Molecular mechanisms of therapy-induced tumor cell plasticity and associated resistance are largely unknown.. Using a genome-wide CRISPR-Cas9 screen, we investigated escape mechanisms of NOTCH-driven TNBC treated with a gammasecretase inhibitor (GSI) and identified SOX2 as a target of resistance to Notch inhibition. Immunohistochemistry:Article Title: Reciprocal inhibition of NOTCH and SOX2 shapes tumor cell plasticity and therapeutic escape in triple-negative breast cancer. Article Snippet: Cancer cell plasticity contributes significantly to the failure of chemoand targeted therapies in triple-negative breast cancer (TNBC).. Molecular mechanisms of therapy-induced tumor cell plasticity and associated resistance are largely unknown.. Using a genome-wide CRISPR-Cas9 screen, we investigated escape mechanisms of NOTCH-driven TNBC treated with a gammasecretase inhibitor (GSI) and identified SOX2 as a target of resistance to Notch inhibition. Transfection:Article Title: Circadian regulation of endoplasmic reticulum calcium response in cultured mouse astrocytes Article Snippet: .. resource Designation Source or reference Identifiers Additional information Gene (Mus musculus) Herpud1 (Herp) Mouse Genome Informatics MGI:1927406 Gene (M. musculus) Bmal1 Mouse Genome Informatics MGI:1096381 Gene (M. musculus) Rev- Erbα (Nr1d1) Mouse Genome Informatics MGI:2444210 Gene (M. musculus) Per2 Mouse Genome Informatics MGI:1195265 Gene (M. musculus) Itpr1 Mouse Genome Informatics MGI:96623 Gene (M. musculus) Itpr2 Mouse Genome Informatics MGI:99418 Gene (M. musculus) Cx43 (Gja1) Mouse Genome Informatics MGI:95713 Strain, strain background (M. musculus, male) C57BL/6 J Charles River Japan N/A Adult PFC experiment Strain, strain background (M. musculus, male and female) B6.129- Bmal1tm1Bra/J Jackson Laboratory Cat# 009100; RRID:IMSR_JAX:009100 Primary Bmal1 KO astrocyte culture Strain, strain background (M. musculus, male and female) 1 day old C75BL/6 N Koatch N/A Primary WT astrocyte culture Transfected construct (M. musculus) CMV- G- CEPIA1er Suzuki et al., Construct:Article Title: Circadian regulation of endoplasmic reticulum calcium response in cultured mouse astrocytes Article Snippet: .. resource Designation Source or reference Identifiers Additional information Gene (Mus musculus) Herpud1 (Herp) Mouse Genome Informatics MGI:1927406 Gene (M. musculus) Bmal1 Mouse Genome Informatics MGI:1096381 Gene (M. musculus) Rev- Erbα (Nr1d1) Mouse Genome Informatics MGI:2444210 Gene (M. musculus) Per2 Mouse Genome Informatics MGI:1195265 Gene (M. musculus) Itpr1 Mouse Genome Informatics MGI:96623 Gene (M. musculus) Itpr2 Mouse Genome Informatics MGI:99418 Gene (M. musculus) Cx43 (Gja1) Mouse Genome Informatics MGI:95713 Strain, strain background (M. musculus, male) C57BL/6 J Charles River Japan N/A Adult PFC experiment Strain, strain background (M. musculus, male and female) B6.129- Bmal1tm1Bra/J Jackson Laboratory Cat# 009100; RRID:IMSR_JAX:009100 Primary Bmal1 KO astrocyte culture Strain, strain background (M. musculus, male and female) 1 day old C75BL/6 N Koatch N/A Primary WT astrocyte culture Transfected construct (M. musculus) CMV- G- CEPIA1er Suzuki et al., other:Article Title: The PAF1 complex promotes 3' processing of pervasive transcripts. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER P-30 column BIO-RAD Cat#7326250 RNA 50 Pyrophosphohydrolase (RppH) NEB Cat#M0356S Hygromycin B InvivoGen Cat#ant-hg-1 Agencourt AMPure XP Beckman coulter Cat#a63881 KAPA Pure Beads KAPABIOSYSTEMS Cat#KK800 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 3X FLAG Peptide Sigma-Aldrich Cat# F4799 Critical commercial assays NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) NEB Cat#E7300S VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina Vazyme Cat#NR603 QuantSeq 30 mRNA-Seq Library Prep Kit REV for Illumina Lexogen Cat#016 VAHTS RNA Adapters set3 for Illumina Vazyme Cat#N809 Deposited data RNA-seq and H3K27ac ChIP-seq data in HCT116 Chen et al., 2017 GEO: GSE97527 GRO-seq data in HCT116 Chen et al., 2015 GEO: GSE70408 RNA-seq data in C2C12 Heinz et al., 2010 GEO: GSE72574 H3K27ac ChIP-seq data in C2C12 Liu et al., 2020 GEO: GSE126147 GRO-seq data in C2C12 Hou et al., 2019 GEO: GSE116169 RNA-seq data in THP-1 Chen et al., 2017 GEO: GSE97527 H3K27ac ChIP-seq data in THP-1 Godfrey et al., 2019 GEO: GSE117864 GRO-seq data in THP-1 Benhammou et al., 2019 GEO: GSE92375 RNA-seq data This paper GEO: GSE162829 ChIP-seq data This paper GEO: GSE162842 3’-seq data This paper GEO: GSE196368 Proteomics data This paper ProteomeXchange: PXD031531 Raw image data Mendeley Mendeley: https://doi.org/10.17632/xgsnsg6cx8.1 PRO-seq data This paper GEO: GSE163501 Experimental models: Cell lines Hela S3 ATCC CCL-2.2 HEK-293T ATCC CRL-11268 PDA cell David et al., 2016 N/A Oligonucleotides See Table S2 for sgRNA and shRNA sequences N/A See Table S3 for qPCR primers N/A Recombinant DNA pLKO5.sgRNA.EFS.tRFP Heckl et al., Article Title: Lymph node colonization induces tumor-immune tolerance to promote distant metastasis. Article Snippet: A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript REAGENT or RESOURCE SOURCE IDENTIFIER C57BL/6J Jackson Laboratories #000664 B6129SF1/J Jackson Laboratories #101043 B6(Cg)-Rag2tm1.1Cgn/J Jackson Laboratories #008449 B6.129S4-Il2rgtm1Wjl/J Jackson Laboratories #003174 B6.FVB-Tg(MMTV-PyVT)634Mul/LellJ Jackson Laboratories #022974 NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ Jackson Laboratories #005557 B6.Cg-Tg(TcraTcrb)425Cbn/J Jackson Laboratories #004194 B6.129S2-Ltatm1Dch/J Jackson Laboratories #002258 B6.Cg-Tg(Tyr-cre/ERT2)13Bos Braftm1Mmcm Ptentm1Hwu/BosJ Jackson Laboratories #013590 B6.Cg-Foxp3tm2Tch/J Jackson Laboratories #006772 B6.129(Cg)-Foxp3tm3(DTR/GFP)Ayr/J Jackson Laboratories #016958 C57BL/6-Tg(TcraTcrb)110OMjb/J Jackson Laboratories #003831 B6;129S-Gt(ROSA)26Sortm1.1(CAG-COX8A/Dendra2)Dcc/J Jackson Laboratories #018397 B6.SJL-Ptprca Pepcb/BoyJ Jackson Laboratories #002014 Oligonucleotides See Table S4 Recombinant DNA lentiCRISPR v2 Sanjana et al., Mutagenesis:Article Title: Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model. Article Snippet: Article Membrane:Article Title: Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model. Article Snippet: Article Electroporation:Article Title: Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model. Article Snippet: Article |